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KMID : 1161520160200050267
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Animal Cells and Systems
2016 Volume.20 No. 5 p.267 ~ p.275
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Fine structural reconstruction on the testicular cyst of the furrow orb weaver, Larinioides cornutus by 3D volume rendering
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Kim Hoon
Seo Jae-Hwi
Kim Kyo-Jin
Chung Kyung-Hoon
Moon Myung-Jin
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Abstract
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Morphological study on spermatids and spermatozoa have long been performed regarding various changes of cell organelles during spermiogenesis as a potential phylogenetic inference. Based on the fact that the number of germ cells per cyst increases according to a geometric series, knowing the exact number of germ cells in a certain stage may lead to the total number of sperms produced per cyst. In spiders, however, the entire process takes place in a cyst represented by a spermatogonium, producing sperms in spherical shape. It is very difficult to count the exact number of germ cells produced per cyst through a 2D image analysis. Therefore, we applied a 3D image of testicular cyst of an orb-weaving spider to visualize the exact number of germ cells produced from a cyst. In this study, 2D images obtained from serially sectioned micrographs were scanned precisely and reconstructed using a 3D-rendering technique. Finally, this research reveals that the exact number of spermatozoa produced each cyst in Larinioides cornutus appeared to be 128 (27), which indicates that a single spermatogonium undergoes five mitotic divisions and two maturing divisions (meiosis) to produce final spermatozoa.
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KEYWORD
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3D reconstruction, testicular cyst, spider, Larinioides cornutus
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